Clinical Assays 2016-11-10T19:42:38+00:00

Notable peer-reviewed articles—using MS to screen a wide range of compounds in biological samples for clinical research applications.

 

Articles of note

The Riddle of ProteinThe riddle of protein diagnostics: future bleak or bright? (free full text)

The theory behind an exploding kaleidoscope of protein biomarker research is that reproducible molecular differences that correlate with important medical attributes can be discovered in patient samples and that these molecules can be measured with clinical tests to obtain useful medical information. How could this not be true? Read more ›

Current role of liquid chromatography coupled to mass spectrometry in clinical toxicology screening methods

Abstract Toxicological screening is the analysis of a biological specimen to detect and identify compounds in patients admitted to the hospital with acute intoxication of unknown origin. The screening of a wide range of toxicologically relevant compounds in biological samples is a serious challenge for clinical laboratories. The high selectivity and sensitivity of liquid chromatography coupled to mass spectrometry or tandem mass spectrometry technology provides an attractive alternative to the current methods. Read more ›

Current and Future ApplicationsCurrent and future applications of mass spectrometry to the clinical laboratory (free full text)

Mass spectrometry is an analytic technique with high specificity and a growing presence in laboratory medicine. Various types of mass spectrometers are being used in an increasing number of clinical laboratories around the world, and, as a result, significant improvements in assay performance are occurring rapidly in areas such as toxicology, endocrinology, and biochemical genetics. This review serves as a basic introduction to mass spectrometry, its uses, and associated challenges in the clinical laboratory and ends with a brief discussion of newer methods with the greatest potential for clinical diagnostics. Read more ›

LC-MS (/MS) in clinical toxicology screening methods

Toxicological screening is the analysis of biological samples to detect and identify unknown compounds. The high selectivity and sensitivity of liquid chromatography (LC) coupled to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) technology provide an attractive alternative to the current methods (LC-UV, GC/MS, etc.). For these reasons, an increasing number of applications are being published. This paper is a brief overview of LC-MS(/MS) screening methods developed for clinical toxicology in recent years.  Read more ›

Mass spectrometry based proteomics profiling as diagnostic tool in oncology: current status and future perspective

Proteomics analysis has been heralded as a novel tool for identifying new and specific biomarkers that may improve diagnosis and monitoring of various disease states. Recent years have brought a number of proteomics profiling technologies. Although proteomics profiling has resulted in the detection of disease-associated differences and modification of proteins, current proteomics technologies display certain limitations that are hampering the introduction of these new technologies into clinical laboratory diagnostics and routine applications. In this review, we summarize current advances in mass spectrometry based biomarker discovery.  Read more ›

Mass spectrometry continues its march into the clinical laboratory

In cartoons, ants often make the sound of battalions of troops walking along deserted roads into battle. If we listen carefully, we might be able hear the same sounds made by mass spectrometers in clinical labs around the world. Indeed, mass spectrometry has changed the face of laboratory medicine and our ability to care for our patients. The method is favored among toxicology and endocrinology laboratories, especially given its long history in the measurement of small molecules. However, several clinical laboratories are beginning to use their mass spectrometers to quantitate peptides and proteins and to detect and identify microorganisms. Read more ›

Quantitative Clinical Chemistry Proteomics (qCCP) using mass spectrometry: general characteristics and application

Proteomics studies typically aim to exhaustively detect peptides/proteins in a given biological sample. Over the past decade, the number of publications using proteomics methodologies has exploded. This was made possible due to the availability of high-quality genomic data and many technological advances in the fields of microfluidics and mass spectrometry. Proteomics in biomedical research was initially used in ‘functional’ studies for the identification of proteins involved in pathophysiological processes, complexes and networks. Improved sensitivity of instrumentation facilitated the analysis of even more complex sample types, including human biological fluids. Read more ›