SRM-based and Other Targeted Methods for Protein Quantification

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SRM-based and Other Targeted Methods for Protein Quantification 2016-11-10T19:41:19+00:00

Research overview–SRM and other targeted methods for protein quantification, formalin-fixed paraffin-embedded (FFPE) tissue, SILAC, and regulatory topics.

 

SRM and other MS-based quantification of proteins

Accurate and reproducible quantification of protein analytes in in complex biological mixtures using selected reaction monitoring (SRM) mass spectrometry and other MS-based techniques continues to grow in popularity. These articles explore the various MS-based quantification approaches. Articles of note ›

Measuring target proteins in formalin-fixed tissue using MS

Recent research shows that formalin-fixed paraffin-embedded (FFPE) tissues offer tremendous potential for the retrospective analysis of the numerous samples stored in hospital archives—all of which include pathological, clinical, and outcome information. These articles explore methods to extract proteins from FFPE tissues for MS analysis. Articles of note ›

SILAC

Many quantitative proteomic analyses involve isotopic labeling of proteins or peptides in experimental groups, which can then be differentiated by MS. SILAC, a widely used isotopic labeling technique, can be applied to complex biomarker discovery and systems biology studies as well as to isolated proteins and protein complexes. As its name implies, SILAC involves labeling protein samples in vivo with a heavy-isotope-labeled form of an amino acid. Inclusion of the labeled amino acid in cell or tissue culture media results in replacement of the natural light amino acid with the heavy form in newly synthesized proteins. These articles explore the SILAC technique. Articles of note ›

CLIA and regulatory aspects of assay development

As biomarker discovery and validation efforts expand, proteomics data will be increasingly submitted to the U.S. Food and Drug Administration (FDA) and other regulatory agencies for review. Currently there are no guidelines describing the generation, submission and assessment of the quality of proteomics data submitted to regulatory agencies. These articles explore potential guidelines and provide definitions, information sources, and data requirements for the various regulatory agencies. Articles of note ›

Clinical assays

Screening the wide range of relevant compounds in biological samples is a challenge for many clinical laboratories. The high selectivity and sensitivity of MS methods is providing an attractive alternative to current methods such as immunochemical assays. For this reason, an increasing number of applications for screening of unknown compounds in biological matrices are being published. These articles provide an introduction to MS along with its use in the clinical laboratory environment. Articles of note ›