Intact Protein Analysis and Variant Mapping

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Intact Protein Analysis and Variant Mapping 2016-11-10T19:27:15+00:00

Research overview—MS-based characterization of intact protein variant forms, amyloidosis, immunoenrichment for MS, and top-down proteomics approaches

 

Immunoenrichment for MS including MSIA

Highly-specific and sensitive mass spectrometry-based immunoassays have been recently established as a suitable alternative to enzyme-linked immunosorbent assays (ELISA) for quantifying proteins in complex biological matrices. Mass Spectrometric Immuno Assays (MSIA) and Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA/iMALDI) are the two most prominent types of MS-based immunoassays. Articles of note ›

Top-Down proteomics

Large-scale proteomics is a mainstay of biological research and clinical translation. Bottom up proteomics strategies using the protease trypsin were the first to be developed and used. However, researchers have begun to find limitations with these strategies–the inability to characterize and quantify intact protein molecules from a complex mixture of digested peptides. To overcome these limitations, researches are attempting to characterize and quantify intact protein molecules: a top-down proteomics approach. These articles review top-down proteomics techniques and how they are being applied to clinical research. Articles of note ›

Amyloidosis diagnostics using MS

Amyloidosis is a heterogeneous group of diseases caused by deposition of mis-folded proteins. Correct identification of the amyloidogenic protein is key to proper treatment. Current methods used for typing of amyloidosis such as immunohistochemistry are helpful but have low specificity and sensitivity. Misdiagnosis can lead to catastrophic therapeutic outcome. Proteomic analysis employing MS-based methods is emerging as a strategy for amyloid typing to identify the causal protein without need for additional clinical information. Articles of note ›