Research Topics

HomeResearch Topics
Research Topics 2016-11-10T18:50:58+00:00

Active areas of MS-based quantitative proteomics research–translational proteomics, targeted methods, companion diagnostics, therapeutic drug monitoring, intact protein analysis and variant mapping.

 

Translational proteomics

The efficient translation of MS-based biomarker discovery research into the development of routine methods is an important challenge in the field of personalized medicine. Bridging this gap requires high-level competence in MS-based biomarker discovery and routine method development for biomarker verification. Understanding clinical research needs and having extensive experience in method verification are also essential to the transformation of novel tests into routine methods. Learn more ›

SRM-based and other targeted methods for protein quantification

MS discovery methods are designed to be qualitative, i.e. to deliver a list of proteins and peptide sequences. In order to assess the biological effects of differential protein abundance in various samples, proteins and peptides must be accurately quantified. Quantification may be done by selected reaction monitoring (SRM) using a triple quadrupole MS or by using a targeted method on a high-resolution instrument. Learn more ›

Biopharma/pharma companion diagnostics and TDM

The therapeutic development pipeline requires monitoring of drug effects and toxicity for clinical trials. Companion diagnostics are assays that measure the levels of proteins or genes in an individual’s sample. Most applications involve metabolite or protein identification and monitoring using MS-based approaches. The goal is to understand and stratify an individual’s disease state in order to select a “personalized” treatment strategy including medication and dose. Learn more ›

Targeted discovery

Traditionally, proteomic discovery experiments are carried out in an unbiased manner using “bottom-up shotgun” techniques. Today, the development of targeted methods has facilitated a more hypothesis-driven approach. Targeted discovery experiments may include specific inclusion mass lists for acquisition by the mass spectrometer. Alternatively, a collection of proteins in a biological pathway may be targeted in a multiplexed SRM assay. Learn more ›

Intact protein analysis and variant mapping

Proteins typically exist in multiple isoforms due to SNP’s, truncations and post-translational modifications (PTMs). Full characterization of a protein includes determination of the protein sequence, and identification and relative quantification of protein isoforms, including identification and localization of one or multiple PTMs. The identification and characterization of clinically relevant protein variants is important and can be accomplished by analysis of intact forms using MS. Learn more ›