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So far Jentech has created 143 blog entries.

SILAC Analysis of Two Bone Marrow Stromal Cell Lines

Within the bone marrow, stromal cells help support hematopoietic cells and inflammatory processes. Two major types of stromal cells related to early cancer development, cell lines HS5 and HS27a, are helpful in studying tumor cell growth and stromal interactions by deciphering crosstalk between stromal cells and clonal cells. According to Li et al., HS5 secretes multiple cytokines that support proliferation of [...]

SILAC Analysis of Two Bone Marrow Stromal Cell Lines

Within the bone marrow, stromal cells help support hematopoietic cells and inflammatory processes. Two major types of stromal cells related to early cancer development, cell lines HS5 and HS27a, are helpful in studying tumor cell growth and stromal interactions by deciphering crosstalk between stromal cells and clonal cells. According to Li et al., HS5 secretes multiple cytokines that support proliferation of [...]

A New Resistance Strategy in Escherichia coli

Selective pressure in response to antibiotic drug treatments is giving rise to drug-resistant and, even more concerning, multi-drug-resistant bacteria. This growing concern is enough to keep epidemiologists up at night. Fortunately, a new class of compounds, antimicrobial peptides (AMPs), use different mechanisms to target pathogens. AMPs and proline-rich AMPs, such as apidaecin 1b, produced in honeybees, show promise in clinical [...]

A New Resistance Strategy in Escherichia coli

Selective pressure in response to antibiotic drug treatments is giving rise to drug-resistant and, even more concerning, multi-drug-resistant bacteria. This growing concern is enough to keep epidemiologists up at night. Fortunately, a new class of compounds, antimicrobial peptides (AMPs), use different mechanisms to target pathogens. AMPs and proline-rich AMPs, such as apidaecin 1b, produced in honeybees, show promise in clinical [...]

The Deep Phosphoproteome: A Stem Cell Data Set

Researchers use human pluripotent stem cells (hPSCs) to model human development and disease, including research related to regenerative medicine and drug discovery. While large-scale hPSC analyses exist, deep characterization of pluripotency, lineage specification and reprogramming via proteomic analysis, especially using advanced technology like label-free quantification (LFQ), remains undone. To this end, Singec et al. (2016) compared proteomic and phosphoproteomic changes [...]

The Deep Phosphoproteome: A Stem Cell Data Set

Researchers use human pluripotent stem cells (hPSCs) to model human development and disease, including research related to regenerative medicine and drug discovery. While large-scale hPSC analyses exist, deep characterization of pluripotency, lineage specification and reprogramming via proteomic analysis, especially using advanced technology like label-free quantification (LFQ), remains undone. To this end, Singec et al. (2016) compared proteomic and phosphoproteomic changes [...]

Profiling Human Islet Cells with Laser Capture Microdissection

Composed mainly of β-cells, human islet cells secrete hormones that help regulate blood sugar. In type 1 diabetes (T1D), the immune system attacks β-cells, causing cell dysfunction and death. While researchers have determined several risk factors to developing T1D, including a genetic predisposition and environmental triggers, the exact pathogenic mechanisms remain unknown. Zhang et al. posit that investigating proteomic changes [...]

Profiling Human Islet Cells with Laser Capture Microdissection

Composed mainly of β-cells, human islet cells secrete hormones that help regulate blood sugar. In type 1 diabetes (T1D), the immune system attacks β-cells, causing cell dysfunction and death. While researchers have determined several risk factors to developing T1D, including a genetic predisposition and environmental triggers, the exact pathogenic mechanisms remain unknown. Zhang et al. posit that investigating proteomic changes [...]

Ebola Vaccines: Liquid Chromatography–High-Resolution Mass Spectrometry for Virus-Like Particle Quantitation

The recent Ebola virus (EBOV) outbreak in West Africa, which took more than 10,000 lives in 2014, highlighted the critical need for robust treatment and prevention options to combat this highly virulent pathogen. One promising area of investigation uses non-infectious virus-like particles (VLPs) to mimic wild type viruses during vaccine construction. This strategy is the same one used to prepare [...]

Ebola Vaccines: Liquid Chromatography–High-Resolution Mass Spectrometry for Virus-Like Particle Quantitation

The recent Ebola virus (EBOV) outbreak in West Africa, which took more than 10,000 lives in 2014, highlighted the critical need for robust treatment and prevention options to combat this highly virulent pathogen. One promising area of investigation uses non-infectious virus-like particles (VLPs) to mimic wild type viruses during vaccine construction. This strategy is the same one used to prepare [...]