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Welcome to the world of translational and quantitative proteomics. This site was developed to provide researchers with useful information and tools for mass spectrometry (MS)-based approaches to protein quantification.

To understand the functions of individual proteins and their place in complex biological systems, it is often necessary to measure changes in protein abundance relative to changes in the state of the system. As such, modern proteomics has evolved from a qualitative technique into one that spans a continuum of qualitative and quantitative approaches.
MS has emerged as a powerful technology for system-wide identification and quantitation of proteins. It can be used for both unbiased discovery-based (untargeted) and targeted determination of changes in protein abundance.

New in translational and quantitative proteomics

New Diagnostic Biomarkers in Colorectal Cancer

Colonoscopies are currently the best method to diagnose colorectal cancer (CRC). A less invasive method, the fecal occult blood test (FOBT), can detect CRC in average-risk individuals; however, it is only 50% effective. With prognosis tied to early diagnostics and a rise in CRC worldwide, researchers such as Peltier et al. seek new diagnostic biomarkers in blood.1   Blood samples for this investigation came from [...]

Analysis of Ketosteroids by Girard P Derivatization

Dehydroepiandrosterone (DHEA); 4-androstene-3,17-dione (AD); and testosterone are androgenic 19-carbon steroids that serve as precursors for other male and female sex hormones. They influence sex-specific and sexually dimorphic processes such as spermatogenesis, muscle growth and neural development. In humans, the adrenal glands primarily produce DHEA, while adrenals and gonads both produce AD. The testes produce 95% of testosterone in males, compared to 50% by the ovaries and adrenal precursors in [...]

Where’s the Squeeze? Informatics Bottlenecks in Missing Protein Identification

The Chromosome-centric Human Proteome Project (C-HPP), an initiative of the Human Proteome Organization (HUPO), seeks to identify all proteins encoded by genes as a bridge between the worlds of genomics and proteomics. However, problems exist in that researchers have not yet identified some predicted gene products. Choong et al. (2015) examined possible informatics issues contributing to this problem through systematic analysis of an [...]

Eye Spy: Direct Visualization of Neurotransmitters

Neurotransmitters (i.e., biogenic amines and amino acids) are common chemical messengers that promote intersynaptic signal transmission, thereby participating in brain function. Direct knowledge of brain structure and function is critical for understanding and treating brain disorders like addiction, depression and neurodegenerative disease. Until now, researchers relied on two non-ideal strategies to acquire this data: indirect analysis, which is labor-intensive and yields low specificity, [...]

Top-Down Proteomic Characterization of Histone H3 Proteoforms in Disease

Zheng et al. performed top-down mass spectrometric proteomic analysis to characterize human histone H3 proteoforms in primary multiple myeloma cell lines.1 Using malignant cells that overexpress histone methyltransferase MMSET, the researchers investigated driver mutations that contribute to modification of histones responsible for epigenetic control in disease. Although researchers understand that specific proteoforms correlate well with disease and that mutations often contribute to pathogenesis, it [...]

Protein Expression Changes During Preeclampsia

Preeclampsia  is a dangerous complication in pregnancy and is potentially life-threatening for both mother and baby. Researchers such as Kim et al. are trying to reduce preeclampsia complications by establishing earlier diagnostics based on blood biomarkers.1 The team used a global proteomics strategy to look for PE candidate biomarkers in three pairs of plasma samples obtained from pregnant women in the early second [...]

Protein Expression Changes During Preeclampsia

Preeclampsia  is a dangerous complication in pregnancy and is potentially life-threatening for both mother and baby. Researchers such as Kim et al. are trying to reduce preeclampsia complications by establishing earlier diagnostics based on blood biomarkers.1 The team used a global proteomics strategy to look for PE candidate biomarkers in three pairs of plasma samples obtained from pregnant women in the early second [...]

Effects of Rosemary Extract on Colon Cancer Cells

  A team of researchers from Spain and Sweden recently examined the effects of rosemary (Rosmarinus officinalis L.) on colon cancer cell proliferation. It may seem like a feeble treatment for something as serious as cancer, but Valdés et al. explain that rosemary has documented health benefits, with its medicinal uses dating back thousands of years. The high antioxidant activity in rosemary comes [...]